Human Plasminogen ELISA Kit - This kit is for the quantitative determination of Human Plasminogen in biofluid samples, such as serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . Plg present in samples reacts with an anti-Human Plg capture antibody coated and dried on a microtiter plate. After washing, an anti-Plg detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of active Plg i. A standard calibration curve is prepared along with the samples to be measured using dilutions of Human Plg. Detection Target Human Plasminogen Reactivity Human Range 1.56-100ng/mL Sensitivity 0.61ng/mL Storage Conditions Store at 4C For RUO.